In the last decade, SFC has been transformed from a niche and esoteric application area into a precise analytical technology for both analytical and preparative chromatography.  It has entered the mainstream for the isolation and purification of chiral and achiral compounds in supporting drug discovery.

You are invited to join our CACA/Shimadzu webinar on Wednesday, September 9. 2020, 12 -1 pm EST.  Please register by using the link below.

Registration link: https://register.gotowebinar.com/register/9051354950157867792

Speaker: Eric Manning, Senior Field Sales Engineer (PA/NJ), Shimadzu Scientific Instruments, Inc.

 

Eric Manning

Webinar Title: A Three-Course Meal with a Tall Glass of Supercritical Fluid

Abstract:

The advantages of modern supercritical fluid chromatography (SFC) are indisputable. SFC can provide significant improvements in many aspects of challenging separations and chromatographic efficiency. However, SFC systems, both analytical and preparative, have historically been beset with many sour traits. Recently, scientists were able to address these deficiencies through collaborative development, rendering SFC a viable and useful analytical and preparative technology.

SFC has recently made significant progress in many application areas, particularly for chiral analysis, and the high-throughput purification of chiral and achiral pharmaceutical compounds in support of drug discovery. The webinar will highlight application and performance data from the Nexera UC series of SFC instrumentation.

Key Learning Objectives:

  • Understanding the advantages and disadvantages of SFC
  • How SFC can be applied to analytical and preparative workflows
  • A novel approach to address specific application needs
  • Modern perspectives on the use of analytical and preparative SFC

Who Should Attend:

  • Method developers for SFC methods in pharmaceutical, foods and cosmetics, chemical, environmental, agrichemical, contract research organization, university, and governmental laboratories.

• Scientists analyzing or purifying chiral and achiral compounds, structural isomers, hydrophobic compounds, or seeking orthogonal selectivity in chromatography. 

Speaker: Kai Wang, Ph.D., Director at Frontage Laboratories, Inc

When: Wed, Aug 5, 2020 12:00 PM - 1:00 PM EDT

Kai Wang

Registration link: https://register.gotowebinar.com/register/5132760379950029840

Abstract: Surrogate matrix has been widely used in bioanalysis, especially for endogenous compounds analysis. Surrogate analyte approaches, on the other hand, are only occasionally employed in bioanalysis, but play a significant role and serve as a useful tool for the quantitation of various analytes, ranging from small molecular biomarkers to large molecule biological entities. This webinar will give a brief review of the surrogate analyte approaches during bioanalysis using LC-MS platform, from both scientific and regulatory perspectives. A few case studies will be discussed in detail laying out the challenges, and approaches to tackle these challenges

 

Key Learning Objectives:

• Bioanalytical Challenges & Approaches for using surrogate analytes

• Successful cases of using surrogate analytes and highlights

• Future work consideration from scientific and regulatory perspectives Who Should Attend:

• Bioanalytical method developers who are interested in using surrogate analytes

 

Who Should Attend:

• Bioanalytical method developers who are interested in using surrogate analytes

Register free: http://www.chromatographyonline.com/lcgc_w/virtual_symposium

Symposium Overview:

 

Session 1:  Tuesday, June 23, 2020: 11am EDT - 1pm EDT
Session 2: Tuesday, June 23, 2020: 2pm EDT - 3pm EDT
Session 3: Wednesday, June 24, 2020: 11am EDT - 12:30 pm EDT

In light of the shelter-in-place situation that has caused the cancellation of numerous in-person conferences, LCGC presents an online symposium on June 23 and 24, 2020. Speakers will discuss high performance liquid chromatography (HPLC) techniques and methods, with a focus on practical implementation. In particular, applications in pharmaceutical, biopharmaceutical, and food analysis will be highlighted, along with a tutorial on two-dimensional LC (2D LC).

The LCGC virtual symposium has been planned in association with the organizers of the HPLC 2020/2022 conference and includes several invited speakers for the HPLC 2020/2022 event.

Registration is free for all participants. 

Sponsors: ACD/Labs, Avantor, Hamilton. Regis Technologies, Shimadzu, and Tosoh Bioscience

Session 1 - Tuesday, June 23, 2020: 11:00 am - 1:00 pm EDT

Pharmaceutical & Biopharmaceutical Analysis

Session chairs: Dwight Stoll, Gustavus Adolphus College, and Zach Breitbach, Abbvie

Session 2 - Tuesday, June 23, 2020: 2:00 - 3:00 pm EDT
2:00 pm EDT
 

TUTORIAL:

Two-Dimensional Liquid Chromatography: A Tutorial with a Focus on Current Best Practices

Dwight R. Stoll, PhD, Gustavus Adolphus College, and Bob W.J. Pirok, PhD, University of Amsterdam

Session 3 - Wedneday, June 24, 2020: 11:00 am - 12:30 pm EDT

Food Analysis

Session chair: Mary Ellen McNally, FMC
 

Speakers: Session chair Dwight Stoll, Professor, Gustavus Adolphus College

Session chair Zach Breitbach, Principal Research Scientist, Abbvie

Laura E. Blue, Principal Scientist, Process Development, Attribute Sciences, Amgen Inc.

Imad Haidar Ahmad, Associate Principal Scientist, Merck & Co., Inc.

James Grinias, Assistant Professor, Department of Chemistry & Biochemistry, Rowan University

Claus Rentel, Executive Director, Analytical Development and Quality Control, Ionis Pharmaceuticals

Time and date:
Session 1:  Tuesday, June 23, 2020: 11am EDT - 1pm EDT
Session 2: Tuesday, June 23, 2020: 2pm EDT - 3pm EDT
Session 3: Wednesday, June 24, 2020: 11am EDT - 12:30 pm EDT

On demand available after airing until Jun. 24, 2021.

Register free: http://www.chromatographyonline.com/lcgc_w/virtual_symposium

Speaker: Ximo Zhang, Ph.D., Senior Scientist in the Biopharma group at Waters

Time: Wed, Jul 1, 2020 12:00 PM - 1:00 PM EDT

Ximo Zhang

Registration link: https://register.gotowebinar.com/register/1003648914833798416

Abstract: Adeno-associated viruses (AAVs) have become the most widely used vectors in gene therapy development for their low toxicity and long-term expression ability. To ensure the safety and quality of gene therapy products, the critical quality attributes (CQAs) of AAVs must be well understood and monitored throughout the product development process. While conventional analytical methods such as analytical ultra-centrifugation (AUC) and SDS-PAGE can provide valuable information regarding the CQAs of AAVs, the results are often laborious to acquire and difficult to validate. Here, we report the development of LC- and MS-based methods for comprehensive characterization of AAV capsids (the protein shells of viruses) in order to deliver efficient, accurate, and reproducible measurement of CQAs, including capsid protein stoichiometry and modifications, aggregation, and empty/full ratio.
 
Key Learning Objectives:
• How to use SEC for AAV aggregation and titer analysis
• Optimize IEX for AAV empty/full capsids analysis
• Develop LC/MS-based method for AAV proteins and peptides analysis Who Should Attend:
• Method developers for LC and LC-MS methods of gene therapy products in pharmaceutical, contract research organization, university and governmental laboratories.
• Analytical scientists analyzing AAV or related biomolecules
 
Who Should Attend:
• Method developers for LC and LC-MS methods of gene therapy products in pharmaceutical, contract research organization, university and governmental laboratories.
• Analytical scientists analyzing AAV or related biomolecules